ABSTRACT
The progress of mainly experimental studies on the chronic toxicity test of the automobile exhaust-involving reproductive system impairment, chronic lung impairment and lung cancer in this paper, and the general situation and the progress on the latest domestic and foreign studies on the possiblely multiple mechanisms of automobile exhaust-induced chronic toxicity are introduced. Some epidemiological study results on the automobile exhausts-induced chronic impairments are reviewed, as well as some problems required to be explored further in the occupational risk assessment of exposure to automobile exhausts in recent years.
ABSTRACT
Objective To observe the toxicity of diesel exhaust particles extracts(DEPE) on V79 cell in cell viability, membrane and oxidative stress. Methods Cell exposed different concentrations of DEPE for different time , the change of cell viability, the leakage of intracellular lactate dehydrogenase(LDH), intracellular glutathione(GSH) and glutathione peroxidase (GPX)content were determined respectively, and analyzed the relation between GSH and GPX. Results The results showed that the viability of V79 cell decreased and the leakage rate of lactate dehydrogenase increased gradually in according with the increasing dosage of DEPE,and that DEPE could impair V79 cell by oxidative stress, included intracellular GSH content decreased and the activity of GPX increased, indicating that GPX might play a important role in DEPE induced GSH depletion. Conclusion DEPE may impair cell viability and the cell membrane integrity, also impair cell by oxidative stress.